Skip to main content
. 2010 May 28;5(5):e10874. doi: 10.1371/journal.pone.0010874

Figure 5. Aberrant cellular phenotypes recover rapidly in PI treated cells following washout of FTI and GGTI.

Figure 5

(A) Labeled with LaA/C antibody, immunofluorescence microscopy of Saos-2 cells reveals that the combination of FTI-277 and GGTI-2147, but not FTI-alone, rectify aberrant nuclear morphology and abnormal nucleoplasmic aggregation caused by Lop. Release of FTI and GGTI enhance this phenotypic rebound. Bar 10µm. (B) Measurements of nuclear circularity and percentage of cells with metaphase and early G1 aggregates reveal that corresponding cellular phenotypes are reversed by 15 hrs following FTI-277 and GGTI-2147 washout and by 24 hrs following FTI-277 washout (error bars = SEM; N = 3, >75 cells counted each experiment).