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. 2010 May 28;5(5):e10891. doi: 10.1371/journal.pone.0010891

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Alkaitis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Confocal analysis was used to determine phospho-p38 (P-p38) cellular localization in the superficial laminae of the ipsilateral L5 dorsal horn in AM281 + AM630-treated rats on day 15 after paw incision. Representative images are shown. Phospho-p38 appears in green. GFAP (marker for astrocytes), ED2/CD163 (ED2, marker for perivascular microglia), Iba-1 (marker for microglia) and NeuN (marker for neurons) appear in red. The color of ED2/CD163, Iba-1 and NeuN staining was digitally changed from green to red, and phospho-p38 from red to green for consistency in presenting the data and in order to allow a better visualization of the co-localization of these markers. The colors of the GFAP/phospho-p38 co-stain (top panel) were not altered.