Table IV.

Association between DNA methylation in ‘normal’ colonic tissues by current use of HT

	Non-current HT use (non-exposed)	Current HT use (exposed)	Adjusted ORa	95% CI
ESR1
Low (≤10)	51	17	1.00
Middle (>10–20)	29	20	0.72	0.29–1.79
High (>20)	83	33	0.99	0.42–2.31
P trend				0.95
ESR2
No (0)	72	28	1.00
Yes (>0)	89	42	0.94	0.49–1.81
P trend				0.85
PGRb
Low (≤5)	79	24	1.00
Middle (>5–10)	35	20	0.55	0.24–1.28
High (>10)	45	26	0.47	0.22–1.02
P trend				0.055
CDKN2A
Low (≤1)	55	21	1.00
Middle (>1–10)	60	33	0.72	0.30–1.72
High (>10)	45	13	1.16	0.39–3.41
P trend				0.78
MGMTc
Low (≤5)	47	19	1.00
Middle (>5–25)	56	38	1.04	0.30–3.69
High (>25)	53	11	3.13	0.75–13.01
P trend				0.025
MYOD1
Low (≤5)	49	18	1.00
Middle (>5–10)	32	17	0.75	0.31–1.80
High (>10)	83	35	0.85	0.40–1.81
P trend				0.72
MLH1
Low (≤0.02)	63	17	1.00
Middle (>0.02–1)	35	24	0.48	0.21–1.08
High (>1)	65	28	0.76	0.35–1.65
P trend				0.59
Total score (ESR1, ESR2 and PGR)d
Low (≤1)	61	19	1.00
Middle (2)	56	26	0.64	0.28–1.43
High (3)	41	25	0.51	0.21–1.25
P trend				0.14
Total score (all seven genes)e
Low (≤3)	40	14	1.00
Middle (4–5)	53	20	1.46	0.53–4.97
High (6+)	55	31	0.63	0.22–1.86
P trend				0.22

^aAdjusted for age, race/ethnicity, batch run and subsite (right/left) of colon.

^bAdjusted OR 1.0, 0.57 and 0.07 (P trend = 0.017) when we used cutpoints of 0–5, 5–50 and >50 PMR in the analysis.

^cAdjusted OR 1.0, 1.25 and 10.6 (P trend = 0.062) when we used cutpoints of 0–5, 5–50 and >50 PMR in the analysis.

^dAnalyses were conducted among those with data on all three genes: the numbers with data in normal tissue analyses were 158 non/ex-HT users and 70 current HT users).

^eAnalyses were conducted among those with data on all seven genes: the numbers with data in normal tissue analyses were 148 non/ex-HT users and 65 current HT users.