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. 2010 Mar 29;31(6):1132–1141. doi: 10.1093/carcin/bgq058

Fig. 1.

Fig. 1.

FOXM1 is upregulated by EPS8. (A) HN4/V and HN4/EPS8 cells were cultured in the absence of serum, RNA prepared, reverse transcribed and used as template in qRT–PCR experiments with primers for the indicated genes. Relative expression is indicated, normalized to actin as an internal standard. Bar = standard deviation. (B) HN4 cells were stably transfected with an EPS8 expression plasmid or empty vector as control, cell lysates prepared and analyzed by western blotting with the indicated antibodies. (C) Saos-2 cells were transiently transfected with an EPS8 expression plasmid or empty vector and analyzed by western blotting with the indicated antibodies. (D) HN12 cells were transiently transfected with small interfering RNA targeting EPS8, or a non-targeting control, and analyzed by western blotting with the indicated antibodies.