Fig. 4.

FOXM1 RNAi inhibits cell growth in EPS8-overexpressing cells. (A) HN12 cells transfected with a non-targeting control (NTC) or FOXM1 shRNA plasmid were lysed and western blotted with FOXM1 antibody or ERK-2 as a loading control. (B) The indicated cells were pulsed with BrdU, then immunostained with anti-BrdU antibodies and counted as described in Methods. Bar = standard error of the mean (SEM). (C). HN4/EPS8 cells were stably transfected with the FOXM1 shRNA plasmid or a non-targeting control. Cell proliferation was determined by daily counting. Bar = SEM. (D) Protein lysates were prepared from the cells described in (C) and western blotted with the indicated antibodies.