Figure 6.

Cellular specificity of the pFIN-IRBP156-CHER-RK-GFP-WPRE dual-promoter vector. pFIN-IRBP156-CHER-RK-GFP-WPRE (1.5×10⁹ vector genomes/µl) lentivirus was injected into the developing neural tubes of E2 chicken embryos in ovo. The retinas of the injected embryos were harvested on E19–20, examined as whole mounts using native fluorescent, and sectioned (10 µm). A-C: A representative section showing the extent of photoreceptor infection. The merged image indicates that nearly all cells are co-expressing the two fluorescent reporter proteins, CHER and GFP. D-F: Close up of transduced photoreceptor layer in region containing INL cells expressing the viral transgene (arrow in D). All sections were counterstained with DAPI. All scale bars shown equal 50 µm. Abbreviations: ONL represents outer nuclear layer; INL represents inner nuclear layer; IPL represents inner plexiform layer.