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. 2010 May 27;16:916–934.

Table 1. PCR primers for lentivirus vector constructs.

Vector insert Primer name Sequence
RK
 
 
 
S-BamHI
TTAGGATCCGGGCCCCAGAAGCCT
 
S-EcoRV
TTAGATATCGGGCCCCAGAAGCCT
 
AS- KpnI
ATTGGTACCGCCCTTGGCCTGTGG
 
AS-PmeI
ATTGTTTAAACGCCCTTGGCCTGTGG
XOPS
 
 
 
S-NheI
ATTGCGGCCGCAGATCTTTATACATTGCT
 
AS-NheI
AATGCTAGCCTCGAGATCCCTAGAAGC
NRLL
 
 
  
S-NotI
TTGCGGCCGCGCGCTACCGGACTCA
 
AS-NheI
GCGCTAGCGGCGACCGGTGGAT
EFIα
 
 
 
S-NotI
ATTGCGGCCGCTTTGGAGCTAA
 
AS-NotI
TTAGCGGCCGCCACGACACCTGAAAT
WPRE
 
 
 
S-SpeI
ATAACTAGTATAATCAACCTCTGGATT
 
S-MluI
ATAACGCGTATAATCAACCTCTGGATT
 
S-KpnI
GGTGGTACCATAATCAACCTCTGGATT
 
AS-SpeI
ATAACTAGTGGTCGACGGTATCGATGC
 
AS-MluI
ATAACGCGTGGTCGACGGTATCGATGC
 
AS-KpnI
ATAGGTACCGGTCGACGGTATCGATGC
mCherry
 
 
 
S-NheI
AAAGCTAGCAAGGATCCCGCCACC
 
AS-NheI,MluI
AAAGCTAGCACGCGTCGTACGTCGGGCTTTGT
GFP
 
 
 
S-NheI
GCAGCTAGCCGCCACCATGAGCAA
 
AS-NheI, MluI
AATGCTAGCACGCGTCGTACGAGAGGCCTCAG
 
S-XhoI
CCTCTCGAGCGCCACCATGAGCAA
 
AS-XhoI
AATCTCGAGAGAGGCCTCAGTCAG
IRBPI56
 
 
 
S-NheI
TTGCGGCCGCGCGCTACCGGACTCA
 
AS-NotI
GCGCTAGCGGCGACCGGTGGAT
RK-GFP
 
 
 
S-SpeI
ATACTAGTGGGCCCCAGAAGCCT
 
AS-SpeI, MluI
 
 
S-XhoI
ATCTCGAGGGGCCCCAGAAGCCT
 
AS-XhoI
ATCTCGAGGATATCTTACTTGTACAGCTCGTCC
tdTomato
 
 
 
S-PmeI
AATGATGTTTAAACCTGTACGACGATGACGATAAG
 
AS-SalI
TTACATGTCGACTCCTTTCGGGCTTTGTTA
Vimentin
 
 
 
S-MluI
GGCACGCGTCTGTTTTACCCACCATCTCAGTTCTAATATT
  AS-MluI GGCACGCGTGGTACCCGGGGATCCACTAGTTCTAGAAAT