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. 2010 Jun;130(2):296–306. doi: 10.1111/j.1365-2567.2009.03235.x

Figure 2.

Figure 2

Invariant natural killer T (iNKT) cell counts and CD69 expression in the liver in early arthritis. Liver mononuclear cells from mice immunized with collagen II in complete Freund’s adjuvant (CII/CFA, n = 6) were collected on day 6 post-collagen-induced arthritis induction and labelled with fluorochrome-conjugated anti-T-cell receptor β (TCR-β) and with either CD1d/α-galactosylceramide (α-GalCer) -tetramer or CD1d/vehicle-tetramer then analysed by flow cytometry. Liver mononuclear cells from non-immunized mice (n = 6) were used as controls. (a) The gate of iNKT cells, defined here as TCR-β+ CD1d/α-GalCer-tetramer-positive cells, among liver mononuclear cells is indicated in each dot-plot for one mouse (representative experiment out of 6). (b) The percentages of iNKT cells among liver MNC are given as mean values ± SEM. (c) iNKT cells were labelled with fluorochrome-conjugated anti-CD69 monoclonal antibody. Results are expressed as mean fluorescence intensity (MFI) corrected for non-specific staining. *P<0·05 versus non-immunized mice (Student’s t-test). Data represent one experiment representative of two similar experiments.