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. Author manuscript; available in PMC: 2010 May 29.

Published in final edited form as: Histochem Cell Biol. 2009 May 13;132(2):191–198. doi: 10.1007/s00418-009-0604-2

Fig. 3 — (A) DAB/hematoxylin dual-color stain of transverse sections of aorta at 7 days after the surgery for denuding the endothelium. Staining was carried out using with anti-CPI-17 and peroxidase-labeled secondary antibody. Right panel shows control experiments without the primary antibody. (B, C) Indirect immunohistochemistry with Cy3- and cy5-conjugated antibodies showing the presence of CPI-17 (B) or MYPT1 (C) in red with SM α–actin in green in transverse sections of aorta before (D0) and at 14 days after the surgery for denuding the endothelium (D14). Asterisk indicates the neointima lesion and SM indicates smooth muscle layers in the media. (D, E) The relative expression level of CPI-17 (D) and MYPT1 (E). Mean values ± SEM were obtained from the ratio of intensity at 20–28 different spots of SM layer and neointima of injured aorta.

Fig. 3 — (A) DAB/hematoxylin dual-color stain of transverse sections of aorta at 7 days after the surgery for denuding the endothelium. Staining was carried out using with anti-CPI-17 and peroxidase-labeled secondary antibody. Right panel shows control experiments without the primary antibody. (B, C) Indirect immunohistochemistry with Cy3- and cy5-conjugated antibodies showing the presence of CPI-17 (B) or MYPT1 (C) in red with SM α–actin in green in transverse sections of aorta before (D0) and at 14 days after the surgery for denuding the endothelium (D14). Asterisk indicates the neointima lesion and SM indicates smooth muscle layers in the media. (D, E) The relative expression level of CPI-17 (D) and MYPT1 (E). Mean values ± SEM were obtained from the ratio of intensity at 20–28 different spots of SM layer and neointima of injured aorta.