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. Author manuscript; available in PMC: 2010 May 29.
Published in final edited form as: J Immunol. 2008 Oct 15;181(8):5189–5193. doi: 10.4049/jimmunol.181.8.5189

Figure 1. Selective nuclear receptor agonists inhibit induction of human TSLP mRNA in response to IL-1β.

Figure 1

(a) IL-1β but not nuclear receptor agonists induce expression of TSLP mRNA. 16HBEo¯ cells were stimulated with 1µM RXR (9-cisRA), LXR (GW3965), PPARγ (GW1929), and GR (dexamethasone) or 1 ng/ml IL-1β for the indicated time course, respectively. ** p < 0.01 comparing TSLP mRNA levels in IL-1β-treated cells as compared to NR agonists alone.

(b) RXR agonist, 9-cisRA, suppress IL-1β induced TSLP mRNA expression. Cells were treated with IL-1β in the absence or presence of 1µM RXR, LXR, PPARα, PPARδ, PPARγ, and GR agonists for the indicated time course, respectively. Cells were harvested and measured for mRNA level by real-time quantitative PCR. TSLP mRNA levels were normalized to GAPDH. Data are the mean ± SD of triplicate data points from a representative experiment. *p < 0.05, statistically significant difference compared with IL-1β treatment.