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. Author manuscript; available in PMC: 2010 May 29.
Published in final edited form as: J Immunol. 2008 Oct 15;181(8):5189–5193. doi: 10.4049/jimmunol.181.8.5189

Figure 4. 9-cisRA blocks recruitment of NFκB to endogenous TSLP promoter and NFκB-dependent genes in response to IL-1β.

Figure 4

(a, c, d) Recruitment of NFκB was inhibited at the NFκB binding site of TSLP gene promoter (a), human iNOS gene (c) and human Defensin-2 gene (d) by 9-cisRA. (b) RXR was not recruited to the RXR binding site at the TSLP promoter in spite of RXR agonist stimulation, but was recruited to BLR1 promoter. 16HBEo¯ cells were stimulated with 1 ng/ml IL-1β in the absence or presence of 1µM 9-cisRA for 1 h, and soluble chromatin preparation was immunoprecipitated with anti-NFκB, anti-RXR antibody or control normal rabbit IgG. Purified ChIP and input DNA were analyzed by real-time quantitative PCR with the primers, respectively. The amount of ChIP DNA was normalized to that of input DNA. The mean value of control antibody before stimulation was arbitrarily defined as 1. Data are the mean ± SD of triplicate data points from a representative experiment.