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. 2010 Apr 1;285(23):17310–17317. doi: 10.1074/jbc.M110.120584

FIGURE 1.

FIGURE 1.

UCP2 and -3 are target genes of PPARγ in 3T3-L1 cells. A, mRNA expression of PPARγ, UCP3, and UCP2 during 3T3-L1 adipogenesis. Levels of mRNA was determined by real-time PCR and normalized to the corresponding TFIIB levels. The experiment was performed in duplicate, and the range is indicated. This experiment is representative of three individual experiments. B, ectopic expression of PPARγ2 in 3T3-L1 preadipocytes induces UCP2 and -3 mRNA expression. 3T3-L1- CAR preadipocytes were transduced with adenovirus expressing PPARγ2 (AdHA-PPARγ2) in the presence of 1 μm PPARγ agonist rosiglitazone, or with control adenovirus containing empty vector (AdEmpty) in the presence of vehicle (DMSO). Total RNA was harvested 8 h after transduction, and the mRNA expression of PPARγ, UCP3, and UCP2 was determined by real-time PCR and normalized to the corresponding TFIIB levels. The experiment was performed in triplicate, and the range is indicated.