FIGURE 3.

PPARγ and RXR binding sites are found in proximity and within the Ucp2 and -3 genes. PPARγ and RXR ChIP-PCR to confirm binding sites identified by ChIP-seq. Chromatin was harvested from cross-linked 3T3-L1 cells on days 0 and 6 of differentiation. ChIP was performed using antibodies against PPARγ or RXR. Occupancy (-fold enrichment above myoglobin promoter levels) at the binding sites found in Fig. 2 and at intermittent control regions was determined by real-time PCR. This experiment is representative of three independent experiments.