FIGURE 4.

Only the PPARγ/RXR binding site in intron 1 of the Ucp3 gene mediates PPARγ transactivation of a heterologous promoter. Approximately 500 bp surrounding the PPARγ/RXR binding sites in Ucp2 and -3 were cloned in front of a SV40 promoter in the pGL3-Promoter luciferase reporter vector and transfected into HEK293T cells together with increasing amounts of PPARγ2 expression vector in the presence of 1 μm rosiglitazone or the vehicle DMSO as indicated. Luciferase levels were normalized to the expression from a β-galactosidase control vector. Results are representative of three independent experiments, each performed in triplicate. Standard deviations are indicated.