FIGURE 1.

FGF2, but not VEGF, trans-activates NOS3 promoter in oFPAEC. A, quiescent cells were treated with or without FGF2 (10 ng/ml) for 18 h. Actinomycin D (5 μg/ml) was then added into the cultures. Total RNA samples were extracted at 0, 3, 6, 9, and 12 h postactinomycin D. NOS3 and 18 S were analyzed using real time quantitative reverse transcription-PCR. Quantitative data are presented as a percentage of relative NOS3 expression at time 0. Data were summarized as mean ± S.E. from three independent experiments. B, cells were transfected with luciferase construct driven by the wild-type sheep NOS3 promoter (−1283/+22) and co-transfected with the thymidine kinase-Renilla luciferase vector. After treatment with FGF2 or VEGF (10 ng/ml) for an additional 24 h in the presence of various concentrations of PD98059, trans-activation of the NOS3 promoter was measured by luciferase reporter gene expressions as a ratio of firefly/Renilla luciferase activities, as described under “Experimental Procedures.” Quantitative data are expressed as mean ± S.E. from three independent experiments. *, p < 0.05 versus untreated control.