FIGURE 10.

FGF2 up-regulates NOS3 expression in HUVEC via AP-1. Subconfluent HUVEC were transfected with a decoy AP-1 CDODN or scrambled ODN and then treated without (C) or with FGF2 (10 ng/ml; F) or PMA (100 nm; P) for 24 h. Cellular proteins were harvested for immunoblotting of NOS3 and β-actin. Representative blots of one typical experiment shown depict three independent experiments using HUVEC from different placentas. Quantitative data are expressed as the mean ± S.E. (n = 3) of -fold control (Ctl) (untreated) value. *, p < 0.05 versus controls. Error bars, S.E.