FIGURE 5.

FGF2, but not VEGF, increases JunB/FRA1 binding to the CRE-like site. A, oFPAEC were treated with or without 10 ng/ml FGF2 (F) or VEGF (V) for 3 h. Supershift using an oligonucleotide probe containing the sheep NOS3 promoter CRE-like element was performed as described under “Experimental Procedures.” Representative supershift EMSA images shown depict similar results from three independent experiments for binding of members of the Jun/Fos families and of CREB and phospho-CREB to the CRE-like element. B, oFPAEC were treated with or without 10 ng/ml FGF2 or VEGF for 2 h and then used for ChIP assays. The real-time PCR signals obtained for IP with specific JunB/Fra1 antibodies were estimated with respect to input DNA, and results are expressed as mean ± S.E. (error bars) (n = 3) of -fold changes over controls (Ctl). *, p < 0.05 versus control.