FIGURE 7.

Role of ERK2/1 in FGF2 induction of AP-1 components in oFPAEC. Serum-starved cells were either pretreated with PD98058 (10 μm) for 30 min followed by FGF2 (10 ng/ml) or treated first with FGF2 and then with PD98058 at 5, 30, and 60 min post-FGF2 treatment. Protein samples were harvested 2 h after FGF2 stimulation and analyzed for c-Jun, JunB, and Fra-1 proteins. Representative blots shown depict one typical experiment. Bar graphs summarize data (means ± S.E. (error bars)) of -fold control values from three independent experiments. Bars with different letters (a versus b versus c) differ significantly (p < 0.05), and bars with two letters do not differ from bars with either letter.