FIGURE 2.

FM is released from TDM in the presence of mycobacterial lysates. A, radio-TLC of the lipids in reaction mixtures of ¹⁴C-labeled M. smegmatis planktonic lysate with either lysates of M. smegmatis biofilms (bf), heat-inactivated biofilm lysates (heat bf), or with BSA is shown. B, radio-TLC of the lipids in the reaction mixture of purified ¹⁴C-labeled cell wall (MsCW) with buffer containing BSA, biofilm lysate (bf), heat-inactivated biofilms lysate (bf heat), and planktonic lysate (plnk) is shown. C, radio-TLC of the reaction mixture as described in panel B is shown except that ¹⁴C-MsTDM was used instead of MsCW as a substrate. D, radio-TLC shows lipids in the reaction mixture of purified ¹⁴C-MsTDM with M. tuberculosis biofilms lysate (bf) and buffer containing BSA. Purified FM was used as a control in panels A–D. TLCs in panel A–D were developed in chloroform:methanol 96:4.