FIGURE 4.

Defective biofilms of mc²155:fbpA mutant. A, 5-day biofilms of M. smegmatis, mc²155 wild-type (wt), mc²155:ΔfbpA, and mc²155:ΔfbpA:pMs6398 (complemented) strains are shown. B, shown is radio-TLC, developed in chloroform:methanol:water (90:10:1), of apolar lipids from M. smegmatis wild type (wt) and ΔfbpA mutant showing incorporation of ¹⁴C into MsTDM. The plot shows the relative amount of MsTDM from the two strains. C, shown is radio-TLC of the apolar lipids from the wild-type and mutant strains developed in chloroform:methanol (96:4) showing ¹⁴C incorporation into FM. The plot shows the relative amount of FM in the two strains. 5-Day biofilms of the strains were labeled for 6 h with [¹⁴C]glycerol, and total apolar lipids equivalent to 50,000 cpm from each sample were loaded and compared with either purified MsTDM in panel B (marked with an arrow) or purified FM in panel C.