FIGURE 7.

Anion substitution alters acid-induced neuronal death. A, representative phase (left) and fluorescent (right) micrographs of cultured hippocampal neurons after 15 min of exposure to pH 7.4 or 5.0 solutions containing either NaCl, NaMeSO₃, or NaSCN. Dead cells were labeled with ethidium homodimer-1 staining and identified by fluorescent imaging. B, sum of the percentage of dead cells per the total number of neurons in each of the indicated anion solutions (n ≥ 355; *, p < 0.01 versus pH 7.4 NaCl; **, p < 0.01 versus pH 5.0 NaCl).