TABLE 3.
Activity of various α1β1 sGC mutants at 37 °C
Values for sGC determined in duplicate. The concentration of YC-1 was 150 μm.
| Mutation | Ligand | Specific activity |
Fold activation |
||
|---|---|---|---|---|---|
| −YC-1 | +YC-1 | −YC-1 | +YC-1 | ||
| nmol cGMP min−1mg−1 | -fold | ||||
| wt | 185 ± 0 | 985 ± 125 | 1 | 5 | |
| NO | 13,282 ± 477 | 26,655 ± 5344 | 72 | 144 | |
| β1 V5Y | 33 ± 12 | 161 ± 65 | 1 | 5 | |
| NO | 1,588 ± 588 | 5,162 ± 2845 | 48 | 156 | |
| β1 F74Y | 433 ± 3 | 1,070 ± 157 | 1 | 2 | |
| NO | 5,004 ± 137 | 5,079 ± 1142 | 12 | 12 | |
| β1 I145Y | 94 ± 11 | 181 ± 67 | 1 | 2 | |
| NO | 1,197 ± 68 | 3,216 ± 448 | 13 | 34 | |
| β1 I149Y | 139 ± 0 | 245 ± 42 | 1 | 2 | |
| NO | 416 ± 23 | 1,829 ± 351 | 3 | 13 | |