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. 2010 Apr 6;285(23):17507–17513. doi: 10.1074/jbc.M109.099515

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Microtubules grown from centrosomes: effects of GDP-tub on microtubular dynamics. A, dynamic parameters of microtubules polymerized from centrosomes and GTP-tub (25 μm) in the presence of increasing GDP-tub concentrations. Standard deviations are represented in parentheses. n, number of events. B, microtubule growth rate versus GDP-tub concentration. C, catastrophe frequency (Catast. freq.) versus growth rate of microtubules assembled with increasing concentrations (18, 22, 25 and 28 μm) of GTP-tub (black squares) or with 25 μm GTP-tub in the presence of increasing GDP-tub concentrations (empty squares). D, microtubule shrinkage rate versus GDP-tub concentration.