FIGURE 5.

HRTs antagonize Notch and TGFβ1 activity in SMC differentiation marker expression. A, primary human aortic SMC were transduced with NotchICD alone (−) or with HRT1 (H1) or HRT2 (H2) co-expression for 3 days before collection of cells for immunoblot analysis. Expression of NICD was verified by detection of epitope tags for N1ICD/N2ICD (V5) or N4 (HA). B, SMC transduced with GFP or HRT1 or HRT2 were grown in the absence (−) or presence (+) of 2 ng/ml TGFβ1 for 48 h before collection for immunoblot analysis. HRT expression was confirmed by detection of the FLAG epitope tag.