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. 2010 Apr 6;285(23):17584–17594. doi: 10.1074/jbc.M110.117416

FIGURE 3.

FIGURE 3.

Adi3 contains a functional NLS. A, leptomycin B treatment. Tomato protoplasts expressing GFP-Adi3 for 14 h were incubated with 20 nm leptomycin B for 90 min followed by incubation with 10 μm Hoechst 33342 for 2 h and visualized by confocal microscopy. Merge, overlay of GFP, Hoechst 33342, and autofluorescence images. Bar, 20 μm; arrowhead, nucleus. B, Adi3 interaction with Xpo1. Xpo1 from tomato was cloned and tested for yeast two-hybrid interaction with Adi3 in the LexA system. C, functional analysis of Adi3 NES and NLS. The indicated GFP fusion proteins were expressed in tomato protoplasts for 14 h, treated with 10 μm Hoechst 33342 for 2 h, and visualized by confocal microscopy. Bar, 20 μm; arrowhead, nucleus. D, expression of GFP NLS and NES fusion proteins in tomato protoplasts as detected by α-GFP Western blot.