Nuclear-localized Adi3 protects against AvrPto-induced cell death. A, PtoR tomato protoplasts or B, pfr-3 tomato protoplasts were transformed with indicated GFP-Adi3 constructs, proteins were expressed for 14 h and transformed with an avrPto construct, samples were taken at the indicated times, and cell was viability determined by Evans Blue staining. Graphs represent the average of three independent experiments. Error bars are standard error. One asterisk indicates significant increase in cell viability compared with AvrPto only expression within each time point (Student's t test, p < 0.05).