FIGURE 3.

Time-dependent increase in the activation of MAPK and transcription factors in AD50 versus AD0 cultures treated with 10,12-CLA. AD0 and AD50 cultures were treated with 50 μm 10,12-CLA (C lanes) or BSA (B lanes) control for 6, 12, or 24 h. Cells were harvested and analyzed for protein expression via immunoblot. Membranes were probed with antibodies targeting phosphorylated and total ERK, JNK, and p38 (A) and P-c-Jun, c-Jun, ATF3, and GAPDH (n = 2) (B). The data in both panels are representative of three independent experiments.