FIGURE 4.

10,12-CLA induces inflammatory gene expression in the PDF and ADF fractions of newly differentiated primary human adipocytes. A, human SV cells were supplemented with DM-1 for 3 d yielding cultures containing ∼30% adipocytes (AD30). Cultures were treated with 50 μm 10,12-CLA or BSA vehicle for 12 h and then fractionated using 6% iodixanol (1.03 g/ml). The lipid-laden ADF was floated, and the PDF was pelleted. B, fractionations were verified by measuring gene expression of AEBP-1, aP2, GAPDH and apm1 using qPCR. C, relative mRNA expression of IL-8, IL-1β, COX-2, GAPDH and ATF3 were investigated using qPCR. Data in B and C were normalized to BSA vehicle in the PDF. Means ± S.E. (n = 3) not sharing a common superscript (a–d) differ significantly (p < 0.05). The data in B and C are representative of three independent experiments.