FIGURE 5.

10,12-CLA-induced inflammatory gene expression is greatest in AD50 cultures in the presence of AD0-containing inserts. A, inserts containing either preadipocytes (AD0) or preadipocytes and adipocytes (AD50) were suspended over 6-well plates containing either AD0 or AD50 cultures. B, next, both wells and inserts were supplemented with 50 μm 10,12-CLA or BSA vehicle control for 12 h, and inflammatory gene expression in the cells in the underlying wells was subsequently analyzed via qPCR. Means ± S.E. (n = 3) not sharing a common superscript (a–e) differ significantly (p < 0.05). The data are representative of two independent experiments.