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. 2010 Apr 8;285(23):17725–17733. doi: 10.1074/jbc.M110.121798

FIGURE 2.

FIGURE 2.

Na+-dependent transient currents in response to voltage jumps are abolished by the T101A mutation. A, EAAC1-specific transient currents measured in EAAC1WT in response to voltage jumps at time 0 from 0 mV to voltages ranging from +60 to −100 mV in 20-mV increments. I(Na+) − I(Na+ + TBOA) represents the current in 140 mm extracellular Na+, from which the current in the presence of 140 mm Na+ + 0.1 mm TBOA (a saturating concentration) was subtracted. TBOA blocks only the EAAC1-specific component of the current response. The extracellular solution contained 140 mm NaMES. The intracellular solution contained 140 mm NaMES and 10 mm glutamate. B, to test for changes in membrane capacitance during the voltage jump protocol, currents were measured before and after TBOA application. Upon subtraction from each other (I(Na+) − I(Na+), these currents show no difference. C and D, similar experiments as in A and B for EAAC1T101A.