FIGURE 7.

Distribution of tubulin and acetylated tubulin between soluble and polymerized fractions of treated HeLa and CG-4 cells. Cells were treated with VBL, TSA, TSA plus VBL, or paclitaxel. The soluble (sol) and polymerized (pol) tubulins were visualized by Western blot using anti-tubulin antibody and quantified by densitometry. The distribution of tubulin between the soluble (empty bar) and polymerized (black bar) fractions is illustrated by a stacked bar chart. For each sample, the total amount of tubulin was calculated by summing the values of the corresponding soluble and polymerized pools. The relative fractions were calculated by dividing the value of each pool by the total amount of tubulin. A representative result of three separate experiments is shown.