FIGURE 7.

DN Rab35 does not alter the endocytic retrieval of KCa3.1 from the plasma membrane. Cells were co-transfected with BLAP-tagged KCa3.1 and either GFP-Rab35 (A) or DN GFP-Rab35 (B), and the channel was labeled with streptavidin-Alexa555, and the cells were imaged at time 0 or 1 h. Whether the cells expressed wild type (A) or DN (B) Rab35, KCa3.1 was initially expressed at the plasma membrane (time 0 h, upper panels). After 1 h at 37 °C (lower panels), KCa3.1 was endocytosed into small endosomes in the presence of both wild type (A) and DN (B) Rab35, identical to what was observed in the absence of Rab35. Nuclei are labeled with DAPI (blue). Projection images from multiple z-sections are shown.