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Overexpression of the MA-nSMase in mammalian cell lines. HEK293 and MCF-7 cells were transfected with an expression construct of mouse MA-nSMase. A, the expression of MA-nSMase was detected by Western blot analysis with a polyclonal antibody against V5. CTL, control. The N-SMase activity of the overexpressed enzyme was detected in both HEK293 (B) and MCF-7 cells (C). The values are expressed as the mean ± S.D. (n = 3).
