TABLE 2.
Equilibrium parameters obtained in the different experiments
Note that some of the experimental errors are very large (e.g. for the fits from chevron plots, Fig. 3, and for kNI from the ligand induced folding experiment, used to calculate the Δ GI-N). The parameters presented here, which are calculated from kinetic parameters, should therefore only be used for a qualitative interpretation. Fitting errors are shown for the equilibrium data.
| Experiment | ΔGD-N | mD-N | ΔGI-N | ΔGD-I | mD-I |
|---|---|---|---|---|---|
| kcal mol−1 | kcal mol−1m−1 | kcal mol−1 | kcal mol−1 | kcal mol−1m−1 | |
| Equilibrium 25 °C (Fig. 2) | 5.0 ± 1.1 | 1.09 ± 0.24 | |||
| Equilibrium 37 °C (Fig. 2) | 3.3 ± 0.3 | 1.26 ± 0.12 | |||
| Chevron 25 °C off-pathway (Fig. 3) | 4.1 | 1.25 | 0.6 | 0.7 | |
| Chevron 37 °C triangular (Fig. 3) | 4.2 | 1.75 | 1.3 | 1.1 | |
| Ligand-induced folding 37 °C (Fig. 5) | 1.8 |