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. Author manuscript; available in PMC: 2010 May 29.
Published in final edited form as: Biochim Biophys Acta. 2009 Oct 14;1802(2):275–283. doi: 10.1016/j.bbadis.2009.10.005

Fig. 3.

Fig. 3

(A) Citrate synthase (CS) activity measurements. Solubilized cells were incubated with oxaloacetate, acetyl coA, and DTNB (5′-dithiobis-(2-nitrobenzoic acid)). Production of CoA-DTNB was detected at 420 nm spectrophotometrically. CS activity was 17% and 24% reduced in D61G and SHP2 knockdown cells compared to controls (n=3; p<0.005, **). (B) ATP content was determined with the bioluminescent method and were 33% and 9% reduced in D61G and SHP2 knockdown cells (n=3; p<0.0001, **). (C) Mitochondrial membrane potential measurements using TMRM via flow cytometry analysis (n=4). TMRM fluorescence was normalized to Mitotracker red fluorescence, which is a membrane potential independent mitochondrial marker in these cells. D61G cells show a 61% reduction in fluorescent signal compared to controls indicating decreased ΔΨm levels (p<0.0001, **).