Figure 1.

(a) SDS-PAGE. Purified HsdR was run on an SDS/12.5 % polyacrylamide gel and stained with Coomassie brilliant blue. The marker lane is labelled accordingly. (b) Dynamic light-scattering measurements of HsdR at 4.5 μM and 10 °C. (c) The published hsdR-gene sequence. (d) The revised hsdR-gene sequence. DNA sequencing revealed an additional nucleotide (red). The resulting frame-shift alters the translated amino acid sequence beyond this point and produces a new stop codon. The published stop codon is shown in yellow, and the new stop codon is shown in cyan. The revised C-terminal amino acid sequence of the R subunit is shown in blue.