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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Mol Cell Neurosci. 2010 Mar 1;44(1):94–108. doi: 10.1016/j.mcn.2010.01.012

Fig. 4.

Fig. 4

Enhanced L-type Ca2+ channel opening increases the effects of membrane depolarization. (A) Images of D2 MSNs in corticostriatal co-cultures treated with 20 mM KCl and ionotropic receptor blockers for 24 hours, in the absence or presence of 1 μM Bay K8644. Bar: upper panels 10 μm; lower panels, 5 μm. (B) Quantification of spine density showing that Bay K8644 treatment decreased spine density in the D2 MSNs depolarized by 20 mM KCl (+K+, median =10.1 n=15; +K++Bay K8644, median=5.9, n=14). (C) Quantification of spine head width showing Bay K8644 treatment decreased the spine size in the D2 MSNs depolarized by 20 mM KCl (+K+, median =0.50, n=336; +K++Bay K8644, median=0.45, n=335; p<0.001, Mann-Whitney Rank Sum Test). (D) Examples of mEPSCs recording from the D2 MSNs treated as in (A). (E) Box plot showing that Bay K8644 treatment reduced mEPSC frequency in D2 MSNs depolarized by 20 mM KCl (+K+, median =3.46, n=16; +K++Bay K8644, median=1.82, n=12). (F) Box plot showing that Bay K8644 treatment had no significant effect on mEPSC amplitude (+K+, median =17.09, n=16; +K++Bay K8644, median=16.82, n=12; p=0.981 Mann-Whitney Rank Sum Test). **p<0.005, ***p<0.001, Mann-Whitney Rank Sum Test.