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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Mol Cell Neurosci. 2010 Mar 1;44(1):94–108. doi: 10.1016/j.mcn.2010.01.012

Fig. 8.

Fig. 8

L-type Ca2+ channel- and calcineurin-dependent induction of Nur77 expression in D2 MSNs in response to membrane depolarization. (A) Images of D2 MSNs treated with 35 mM KCl and ionotropic receptor blockers for 24 hrs in absence or presence of nimodipine or Ascomycin/Cyclosporin A. Cultures were stained with anti-GFP antibody (Green), anti-Nur77 antibody (red) and 4 ,6 -diamidino-phenylindole (DAPI, blue). (B) A representative image at a focal plane (1 micron thick) through the soma of a depolarized cell marked in (A) showing that most of Nur77 staining is localized in nucleus. (C) Quantitative analysis of Nur77 staining in the nuclei of D2 MSNs showing that KCl treatment increases intensity of Nur77 staining (control, median=4318, n=99; +K+, median=9206.5, n=198), and nimodipine or Asc/CsA blocks the depolarization-induced Nur77 increase (+K++nimodipine, median=3014.5, n=198; +K++Asc/CsA, median=1876.5, n=104). *** p<0.001, Mann-Whitney Rank Sum Test. Scale bar: low magnification images, 10 μm; high magnification images 5 μm.