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. 2010 Jun 1;24(11):1106–1118. doi: 10.1101/gad.1903310

Figure 4.

Figure 4.

YAP is induced during iPS reprogramming and enhances iPS induction. (A) YAP expression is elevated during iPS reprogramming. Human fibroblasts were infected with OSK and c-Myc to produce iPS as documented. The iPS properties were characterized by cell morphology and staining for pluripotent stem cell markers TRA-1-81 (red) and Nanog (green). (B) Karyotype of iPS cells generated from human fibroblast (Fibro-27). (C) YAP expression in the parental fibroblasts and iPS cells were determined by Western blotting. (D) iPS cells generated from human fibroblasts (Fibro-27) can be differentiated successfully to neural stem cells (NSCs) stained by Nestin (green, left panel) and neurons stained by βIII-tubulin (red, right panel), MAP-2 (green), and DAPI (blue). (E) YAP increases iPS induction. Mouse embryonic fibroblasts (MEFs) were infected by three factors (OSK) in the presence or absence of YAP coinfection. (Top panel) Nineteen days after viral infection, cells were stained for ALP. (Bottom panel) The Oct4-GFP reporter expression was examined to verify iPS induction (note the high magnification to show GFP-positive colonies). Expression of ALP and GFP were quantified by ALP-positive (ALP+) and GFP-positive (Oct4-GFP+) colony numbers.