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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Biochem J. 2010 Apr 28;428(1):125–131. doi: 10.1042/BJ20100122

Figure 1. UV-visible and EPR spectra of purified human IscA homologue hIscA1.

Figure 1

HiscA1 was purified from E. coli cells grown in the LB growth medium. A) UV-visible spectra of hIscA1. Purified hIscA1 (50 µM) (spectrum 2) was incubated with EDTA (10 mM) and L-cysteine (2 mM) at 37°C for 60 min (spectrum 1). The iron-depleted hIscA1 (50 µM) was re-incubated with Fe(NH)2(SO4)2 (50 µM) and dithiothreitol (2 mM) (spectrum 3). Each protein was re-purified by passing through a High-Trap Desalting column after incubation. B) EPR spectra of hIscA1. The protein samples were prepared as described in A), except that the protein concentration was ~450 µM.