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. Author manuscript; available in PMC: 2010 May 31.
Published in final edited form as: Nat Cell Biol. 2007 Dec 9;10(1):53–60. doi: 10.1038/ncb1668

Figure 3.

Figure 3

PRK1 controls modifications of histone H3 and AR-dependent gene expression. For ChIP (a, b) and transient transfections (c, d, e), cells were cultivated in the presence or absence of the AR agonist R1881 and the inhibitor Ro318220 (b, c, d), or transfected with siRNA (a). ChIP analyses were performed with the indicated antibodies. The precipitated chromatin was amplified by PCR using primers flanking AREs in the promoter region of the PSA and KLK2 genes. For transient transfections, CV1 (c, e) or 293 (d) cells were co-transfected with AR expression plasmid and AR-dependent reporters. Bars represent mean +SD (n=4).