A, B and C Apoptotic cell death after combined treatment with TRAIL+PPP, TRAIL+CHX , TRAIL+CHX+PPP or TRAIL+CHX+LY294002 in indicated normal and cancer (melanoma) cell lines. TRAIL (50 ng/ml), CHX (4 µM) and LY294002 (50 µM) and indicated doses of PPP were used with. Error bars represent mean ± S.D. for four independent experiments (Student’s t test, P < 0.05). D. Western blot analysis of active and total AKT levels in LU1205 cells 6 h after treatment with LY294002 (50 µM).