Figure 1. Vβ Use by Retrogenic Treg and Tconv Cells.
CD4+ T lymphocytes from retrogenic mice, preimmune or 22–28 days after EAE induction, were flow cytometrically analyzed with a panel of TCR Vβ-specific antibodies. Comparisons were made between Tconv (A) and Treg (B) cells from unimmunized and immunized splenocytes and CNS T cells from mice with EAE. A significant increase in Vβ8.1 or 8.2 splenocytes was seen among Tconv cells (p = 0.018), and a decrease in Vβ8.3 TCR among Treg cells (p = 0.048) when comparing pre- or immune splenic and CNS-infiltrating populations. Data show mean + 1 SEM. *p < 0.05 among group by Hochberg-adjusted 1-way ANOVA.