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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Placenta. 2010 Apr 24;31(6):528–534. doi: 10.1016/j.placenta.2009.12.010

Figure 1. LAT1 gene expression in TS cells cultured in media containing various concentrations of leucine.

Figure 1

A) The effect of leucine availability on LAT1 mRNA expression was measured by real time PCR. Trophoblast stem (TS) cells were cultured in media containing final leucine concentrations of 20, 40, 60, 80, and 100microM. 800 microM is found in complete medium. Cells were cultured for 24 or 48 hrs. LAT-1 is expressed relative to GAPDH by the Delta Delta CT method. LAT1 mRNA expression was highest in TS cells cultured in 40 µM leucine medium. B) Protein expression was measured by Western blot. Protein expression in TS cells cultured in 40 microM leucine compared to complete medium containing 800 microM leucine. All experiments were carried out at least three independent times with similar results. Values are the means ± SEM. Values from a representative experiment are shown in B. Bars with different letters represent significant differences in LAT1 gene expression among the experimental conditions (p≤0.05).