Table 1.
UAS-Trh induced spheroids are specific to serotonergic neurons
GAL4 driver | UAS-construct | 5-HT Spheroids |
Spheroids in GAL4- expressing cells |
---|---|---|---|
Trh-GAL4 | UAS-Trh | + | + |
5-HT cells | UAS-DDC | − | − |
UAS-TH | − | − | |
th-GAL4 | UAS-Trh | − | − |
DA cells | UAS-TH | − | − |
eg-GAL4 | UAS-Trh | − | − |
5-HT cells early | |||
RN-2-GAL4 | UAS-Trh | − | − |
motor neurons | |||
109(2)80-GAL4 | UAS-Trh | − | − |
motor neurons |
Representative images following these manipulations are presented in Supplemental Figure 3.