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. Author manuscript; available in PMC: 2011 May 28.
Published in final edited form as: Immunity. 2010 Apr 29;32(5):692–702. doi: 10.1016/j.immuni.2010.04.010

Figure 1. TLR expression in Th17 cells.

Figure 1

(a) Expression of various TLRs on Th1, Th2, and Th17 cells following 4-day differentiation. Bone marrow-derived macrophages and IL-17-producing γδ T cells were utilized as positive controls. Relative mRNA expression was compared by setting the lowest expression amount to an arbitrary value of 1. (b) Expression of TLRs on αβ and γδ T cells following IL-23 timepoint stimulation. 20 ng/ml IL-23 was added to sorted splenic CD4+ and γδ T lymphocytes and cells were lysed at 0, 2, 4, 6, 8, 16, and 24 hrs post IL-23 stimulation. Unstimulated cells served as the expression baseline. For all experiments, data are presented as mean + SD and are a representative of at least two independent experiments. All gene quantities were normalized to the expression of the reference gene β-actin.