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. Author manuscript; available in PMC: 2011 May 28.
Published in final edited form as: Immunity. 2010 Apr 29;32(5):692–702. doi: 10.1016/j.immuni.2010.04.010

Figure 2. Enhanced Th17 differentiation following TLR2 activation.

Figure 2

(a) Intracellular cytokine staining following four-day Th17 differentiation in the absence (left) or presence (right) of Pam3Cys. Each panel represents cells derived from an individual mouse, 2 mice were used for each treatment group. (b) ELISA analysis of supernatants from primary four-day differentiation (left) or overnight anti-CD3 only restimulated (right) cultures. All groups are analyzed in triplicate and are presented here as the mean + SD from three individual mice per group. (c) Th17 differentiation was performed as in (a) using naïve T cells derived from WT and Tlr2−/− animals. (d) mRNA expression of naïve and Th17 cells differentiated in the absence or presence of Pam3Cys. Each bar represents mean + SD values obtained from T cells of three individual mice. Naïve T cell gene expression was used as the baseline of expression and all gene quantities were normalized to the expression of β-actin. * = Students t test; p < 0.05. All data are a representative of at least three individual experiments.