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. Author manuscript; available in PMC: 2011 May 28.
Published in final edited form as: Immunity. 2010 Apr 29;32(5):692–702. doi: 10.1016/j.immuni.2010.04.010

Figure 4. The effect of TLR2 activation in combination with IL-23 on αβ and γδ T cells.

Figure 4

(a) Overnight anti-CD3 activation of Th17 cells with various TLR ligands following 4-day differentiation. The production of IL-17 (top) and IL-22 (bottom) was assessed in triplicate by ELISA. * = Students t test, p < 0.05. * = significant comparison of samples without IL-23 to untreated control. ** = significant comparison of samples treated with IL-23 to untreated IL-23 samples and untreated controls. (b) CFSE labeling of sorted naïve CD4+, memory CD4+, and γδ T cells following 2-day stimulation with TLR2 agonist or IL-23. Data are representative of three individual experiments. (c) CFSE labeling of sorted total CD4+ T cells (left) and γδ T cells (right) derived from WT (top) and Tlr2−/− (bottom) animals. Data are a representative of three independent experiments. Numbers shown in each gate are indicative of mean fluorescent intensity.