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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Exp Cell Res. 2010 Mar 27;316(12):1958–1965. doi: 10.1016/j.yexcr.2010.03.018

Figure 3.

Figure 3

Comparison of nmMyH IIB with sarcomeric myosins. Cross-sections of rat orbits were stained with anti-nmMyH IIB antibodies and counterstained with monoclonal antibodies A4.951 (adult slow in A), N2.251 (slow/fast 2A in B) or 2F7 (fast 2A in C). The fibers that exhibit strong staining for nmMyH IIB also exhibit strong staining for the anti-slow sarcomeric MyHC (note arrows in A and B). Additional fibers in B stain with N2.261 are presumably due to the cross reactivity of this antibody with the MyHC fast 2A isoform. The intrafiber staining of nmMyH IIB did not coincide with fibers expressing fast sarcomeric isoforms. Arrows indicate the position of the same fibers in all three panels. Scale bar =10 μm.