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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Exp Cell Res. 2010 Mar 27;316(12):1958–1965. doi: 10.1016/j.yexcr.2010.03.018

Figure 6.

Figure 6

Comparison of the distribution of nmMyH IIB (pseudo blue) with the distribution of slow sarcomeric MyHC (red), actin (green) and nuclei (grey). Muscle in panel A was relaxed and flash frozen prior to sectioning, while muscle in panel B was fixed in 4% formaldehyde prior to processing. In both cases, the antibodies to nmMyH IIB label a distinct strip in the middle of the A-bands containing slow sarcomeric myosins (note arrows in both panels). The observed A-band distribution of nmMyH IIB is independent of fixation conditions. Scale bar = 5 μm.